The human ovarian follicular fluid level of interleukin-8 is associated with follicular size and patient age.
نویسندگان
چکیده
OBJECTIVE To investigate the relationship between interleukin-8 (IL-8) in the human ovarian follicle and follicular size, patient age, and fertility factors in IVF cycles. DESIGN Prospective study. SETTING University hospital research laboratory and infertility clinic. PATIENT(S) Women undergoing IVF with oocyte retrieval. INTERVENTION(S) Follicular fluid (FF) aspiration, oocyte isolation, FF storage, and experimental studies. MAIN OUTCOME MEASURE(S) Quantization of IL-8 by ELISAs and protein microarray; high-performance liquid chromatography (HPLC) followed by ELISA and Western blotting to evaluate alpha(2)-macroglobulin (alpha(2)M) bound IL-8; association of IL -8 to follicular size, patient age, and IVF outcomes. RESULT(S) Samples of FF from 63 patients contained an average of 629.59 pg/mL of IL-8 with 50%-70% bound to alpha(2)M. Large follicles contained higher levels of IL-8 than small follicles (937.34 vs. 86.97 pg/mL). The IL-8 concentration in the large follicles of women of young age was higher than that of older reproductive age women (1,373.61 vs. 673.29 pg/mL). There were no statistically significant associations found between IL-8 concentration and other IVF cycle factors or pregnancy outcome. CONCLUSION(S) Our findings indicate that IL-8 is present in FF, both in its free and alpha(2)M-bound state, and its concentration is correlated with follicular size and patient age.
منابع مشابه
P-77: Follicular Fluid Concentrations of Interleukin-6, Interleukin-8, TNF-α and Interleukin 10 in Polycystic Ovarian Syndrome Women
a:4:{s:10:"Background";s:504:"While the etiology of PCOS has continued to remain a mystery, there can be found some evidence in support of the presence of chronic low-grade inflammation in women with this syndrome. One of the most significant manifestations of chronic inflammation is Pro-inflammatory cytokines. Therefore, the aim of this prospective study was to investigate the pattern of proinfla...
متن کاملP-45: Predictive Value of Follicular Fluid VitaminD on Assisted Reproductive TechniqueOutcome
Background: Vitamin D, a steroid hormone, has critical roles in human health. The available data thus identify vitamin D as an important substance in processes involved in reproductive success and thereby suggest pathophysiological mechanisms for reproductive compromise in the setting of vitamin D deficiency. Realizing the prevalence of vitamin D insufficiency in our country, we hypothesized th...
متن کاملO-25: Relation between Follicular Fluid Levels of 25 Hydroxy Vitamin D and Clinical Pregnancy following Embryo Transfer
Background: Animal and in vitro studies have shown that 25-OH vitamin D plays a significant role in different parts of body. Few data exist about the role of 25- OH vitamin D in reproduction. Insufficient levels during pregnancy are potentially associated with increased risk of preeclampsia, insulin resistance and gestational diabetes mellitus. Recently, few studies have assessed the prognostic...
متن کاملI-21: The Expression of TLRs in Follicular Cells of Poor Ovarian Responder
Background: One of the most annoying problems in IVF is poor ovarian response. It is anticipated that 5-18% of all IVF cycle are affected by poor response to ovarian hyperstimulation. Poor response to goandotropin may lead to decline in the pool of embryos for transfer or cryoperservation, and decrease pregnancy rates. Different mechanisms explain poor ovarian response for example decreased num...
متن کاملFatty Acid Composition of Human Follicular Fluid Phospholipids and Fertilization Rate in Assisted Reproductive Techniques
Background: Fatty acids are known to be critically important in multiple biological functions. Phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women’s fertility and the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of fatty acid composition of follicular fluid ph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Fertility and sterility
دوره 93 2 شماره
صفحات -
تاریخ انتشار 2010